At 400 yards you will see the nodes whether the rifle is .5 moa or 2 moa. Wont matter, but you just might need a bigger target to catch all the shots for the less accurate rifle. You will see the vertical dispersion and where it is minimized either way. More distance generally makes it more obvious. You do not really need to worry about "grouping," as the horizontal spread is not really as telling as the vertical. We are looking for the points, nodes, where the barrel is most stable in its vibration/travel, meaning the top or bottom of its wave movement. If you catch it at these points the and the bullet exits the barrel at that time, the rifle will shoot groups with less vertical spread and generally will be the most accurate.
Once you find what looks like nodes, load em up and see. After that mess with seating depth a little bit to fine tune things. Voila, should be shooting well at this point.
Thats how I approach it at least lol.
Mind you this all assumes the rifle, optic, firing position, ammunition, weather, and shooter are all ON. You cannot shoot a good ladder test unless you are shooting well, otherwise you end up chasing your tail.