Hi WildWillie,
In one of my previous articles related to tuning your crossbow, I made mention that I found it necessary to use a piece of serving o dental floss to lock the serving inside the D-Loop in place. I believe I call in tying the D-Loop in place to prevent any rotation or movement.
The serving that you are referring to can be screwed left or right when you are paper tuning your crossbow. The problem is that if you don't lock it in place, it will continue to move as you shoot from the momentum of the cable on arrow launches. This could easily change your center shot and undo the paper tuning. My suggestion is that you make sure this serving is in its proper place and then lock it down by serving both the left and right sides of it with a small piece of waxed floss or serving.
On the two group problem I would first make sure that my Optimizer Base Screws and my Scope Rings are good and tight. If that doesn't solve the problem, I'v had this happen to me before I added the check rest to my stock. I was having a similar problem, but mine was coming from not aligning the stock to the exact same spot on my face each time. It turned out to be affecting how close my eye was to the scopes lens. This was costing me a couple of inches of elevation at 60 yards or greater. I haven't had the problem since I added the check-rest.
Jon